Mapping the interaction surface of scorpion β-toxins with an insect sodium channel
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منابع مشابه
Interaction of Scorpion α-Toxins with Cardiac Sodium Channels
The effects of the scorpion alpha-toxins Lqh II, Lqh III, and LqhalphaIT on human cardiac sodium channels (hH1), which were expressed in human embryonic kidney (HEK) 293 cells, were investigated. The toxins removed fast inactivation with EC(50) values of <2.5 nM (Lqh III), 12 nM (Lqh II), and 33 nM (LqhalphaIT). Association and dissociation rates of Lqh III were much slower than those of Lqh II...
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Scorpion β-toxins bind to the voltage-sensing domain of voltage-gated sodium (NaV) channels and trap the voltage-sensing domain in the activated state. Two structurally similar β-toxins from scorpions, Css4 and Cn2, selectively target different subtypes of mammalian NaV channels. While the receptor site on the channels is known, the functional surface of the toxins remains to be understood. Her...
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Site-directed and monoclonal antibodies recognizing different extracellular regions of the RII sodium channel alpha subunit have been used to determine the sequences that comprise the receptor for alpha-scorpion toxins by evaluating the effect of antibody on voltage-dependent binding of radio-labeled toxin isolated from Leiurus quinquestriatus to both reconstituted rat brain sodium channel and ...
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Lee, Daewoo, Michael Gurevitz, and Michael E. Adams. Modification of synaptic transmission and sodium channel inactivation by the insect-selective scorpion toxin LqhaIT. J. Neurophysiol. 83: 1181–1187, 2000. The peptide LqhaIT is an a-scorpion toxin that shows significant selectivity for insect sodium channels over mammalian channels. We examined the symptoms of LqhaIT-induced paralysis and its...
متن کاملModification of synaptic transmission and sodium channel inactivation by the insect-selective scorpion toxin LqhalphaIT.
The peptide LqhalphaIT is an alpha-scorpion toxin that shows significant selectivity for insect sodium channels over mammalian channels. We examined the symptoms of LqhalphaIT-induced paralysis and its neurophysiological correlates in the house fly (Musca domestica). Injection of LqhalphaIT into fly larvae produced hyperactivity characterized by continuous, irregular muscle twitching throughout...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 2021
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bcj20210336